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The dose-response curves for atovaquone (ATQ), proguanil (PG) and the constant-ratio combination of the two drugs (a). Triplicate samples were used to derive error bars based on standard error of the mean (SEM).Ĭonstant-ratio combination of atovaquone and proguanil. The parasitaemia of drug treated samples was determined relative to untreated controls (100% parasitaemia). SG-FCM method was used to analyse parasite growth.
#Calcusyn serial series#
(c) Ring stage parasites were treated for 72 hours with a 3-point constant-ratio dose series of atovaquone and proguanil either alone or in combination. (b) Was a replica of (a) but with an increased atovaquone dose series. The mid-point was equated to the previously determined ED 50 value for each drug. (a) Trophozoite stage parasites (strain K1) were treated with a 2-fold dose series of atovaquone (ATQ), proguanil (PG) or a constant-ratio combination of both drugs (ATQ+PG) for 48 hours. Optimisation of the atovaquone-proguanil treatment regime. The dose-effect curve (a), the median-effect plot (b) and the isobologram (c) from the CalcuSyn output are shown alongside the FIC isobologram (d) For the ED 50 ED 75 and ED 90 levels of inhibition.ĬalcuSyn output for the dihydroartemisin-emetine combination. An isobologram was also prepared manually from the same, constant-ratio (ED 50: ED 50), dose-response data based on ∑FIC analysis. Dose-effect analysis was completed in accordance with Chou Talalay method using CalcuSyn. Dose ranges for individual and combined drugs were completed in triplicate.
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Parasites were treated with a dose series of chloroquine, emetine and the combination. Standard error bars are based on the standard error of the mean (SEM) for triplicate data.Ĭomparison of dose-effect and FIC analysis for the dihydroartemisinin-emetine constant-ratio combination. Synchronised trophozoite stage parasites were treated with a two-fold dose series of the test compounds and analysed at 48 hours using the SYBR Green flow cytometer method. The dose series used for the combination of existing antimalarials (AM) with emetine (Eme).ĭose-response curves and ED 50 values for a. Combinatory index values, recommended symbols and descriptions for classifying synergism or antagonism using the Chou-Talalay method.